產(chǎn)品名稱：金氏B培養(yǎng)基

Product name：King’s B medium

產(chǎn)品用途：用于銅綠假單菌產(chǎn)熒光色素試驗(yàn)。

Product usage: Used for the test of producing fluorescent pigment by Pseudomonas aeruginosa.

檢驗(yàn)原理

Test principle

蛋白胨提供氮源；甘油提供碳源；磷酸鹽促進(jìn)熒光素的產(chǎn)生并抑制綠膿色素的產(chǎn)生；硫酸鎂為熒光素的產(chǎn)生提供必須的陽離子；瓊脂是培養(yǎng)基的凝固劑。

Peptone provides nitrogen source; Glycerol provides carbon source; Phosphate promoted the production of fluorescein and inhibited the production of pyocyanin. Magnesium sulfate provides the necessary cation for the production of luciferin. AGAR is a coagulant for medium.

配方成分

Formula ingredients

使用方法

Usage

稱取本品干粉38g，加入蒸餾水或去離子水1L，并加入10mL甘油，攪拌加熱煮沸至完全溶解，分裝試管，每管5mL，121℃高壓滅菌15分鐘后，取出，冷卻培養(yǎng)基，制成斜面。

Weigh the dry powder 38g, add distilled or deionized water 1L, and add 10mL glycerol, stirring and heating to boil until completely dissolved, divided into test tubes, 5mL per tube, 121 ℃ autoclave sterilization for 15 minutes, take out, cool the medium, made of slant.